FastQCFastQC Report
Tue 27 Sep 2022
EGAF00004457770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55564351
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGC49766348.956523221156672TruSeq Adapter, Index 23 (97% over 38bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA2575870.4635832064339238No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA1751430.3152074969794932No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGCC982680.17685440076497969TruSeq Adapter, Index 23 (97% over 37bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT965930.17383987801819192No Hit
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCAACTAATCTCGTATGC890060.16018543976154784TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCAACTAATCTCGTATGC846230.15229728859786376TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCAACTAATCTCGTATGC833880.150074640483068TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCAACTAATCTCGTATGC818020.14722029237775133TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCAACTAATCTCGTATGC766630.13797155661909918TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATCAACTAATCTCGTATGC744620.13401038374406643TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAAGCTCGTATGC744550.13399778573855745TruSeq Adapter, Index 23 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAACTAATCTCGTATGC742610.1336486410144519TruSeq Adapter, Index 10 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCAACTAATCTCGTATGC733100.1319371119803055TruSeq Adapter, Index 7 (97% over 35bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA721480.1298458430658175No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAAACTCGTATGC697430.12551752831595206TruSeq Adapter, Index 23 (97% over 38bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACCAACTAATCTCGTATGC688670.12394097791225889TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATTAATCTCGTATGC622880.1121006524489056TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)