FastQCFastQC Report
Sun 2 Apr 2023
EGAF00004457771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82736439
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCCGAATCTCGTATGC47896805.789081640315702TruSeq Adapter, Index 6 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA5843700.706303059526166No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA3739890.452024530569898No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA2205900.266617711211864No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT1923300.23246105624632937No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCCGAAACTCGTATGC1303400.15753639094861213TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCCGAAGCTCGTATGC1053010.12727282110848404TruSeq Adapter, Index 6 (97% over 36bp)
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT1032420.12478419575200717No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCCGAACCTCGTATGC1026450.12406262735093058TruSeq Adapter, Index 6 (97% over 36bp)
CGGGTTTATGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA1008810.1219305558944832No Hit
GATCGGAAGAGCACATGTCTGAACTCCAGTCACTCCCGAATCTCGTATGC914820.11057038604235796TruSeq Adapter, Index 3 (97% over 34bp)
TGGGTTTATGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA846950.10236722902710377No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)