FastQCFastQC Report
Wed 14 Sep 2022
EGAF00004457773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75954654
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATGC20289502.671264883913499TruSeq Adapter, Index 1 (97% over 37bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA5211960.6861936333749872No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA3221380.42411884332986366No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT2198620.2894648167313092No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA1887770.2485390822792768No Hit
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT1068730.1407063219588888No Hit
TGGGTTTATGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA891570.1173818789300258No Hit
CGGGCGTGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT838240.11036058435602905No Hit
CGGGTTTATGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA830960.10940211774251515No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)