FastQCFastQC Report
Sun 2 Apr 2023
EGAF00004457776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75694073
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGC8187871.0817055649786478TruSeq Adapter, Index 23 (97% over 38bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA4922580.6503256866624154No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA3168870.41864176076243115No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT2152950.28442781775001064No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA1822350.2407520070957207No Hit
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT971350.12832576732923331No Hit
TGGGTTTATGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA914940.12087340048407753No Hit
CGGGTTTATGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA790930.10449034761281772No Hit
CGGGCGTGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT786190.10386414270507018No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)