FastQCFastQC Report
Thu 5 Jan 2023
EGAF00004457777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74455310
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTCGTATGC46268476.214260608141984TruSeq Adapter, Index 3 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA5879210.7896293763332661No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA3653700.4907238986715656No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT1899180.25507650159538653No Hit
GATCGGAAGAGCACGCGTCTGAACTCCAGTCACTCATTCATCTCGTATGC1748080.2347824486930482TruSeq Adapter, Index 3 (97% over 34bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA1658680.2227752459831273No Hit
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT1200120.16118662322405214No Hit
CGGGTTTATGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA920540.12363658146074472No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCACCTCGTATGC871070.11699232734374485TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACTCATTCATCTCGTATGC859180.11539539624507641TruSeq Adapter, Index 3 (97% over 34bp)
CGGGCGTGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT839490.11275085685628064No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCAACTCGTATGC798790.1072844905218983TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACTCATTCATCTCGTATGC755560.10147832303700033TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACTCATTCATCTCGTATGC744750.10002644539388796TruSeq Adapter, Index 3 (97% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)