FastQCFastQC Report
Wed 14 Sep 2022
EGAF00004457778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69544122
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATGC64159819.225770367767387TruSeq Adapter, Index 10 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGAACTCGTATGC1943350.2794413020269348TruSeq Adapter, Index 10 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGACCTCGTATGC1662530.23906118190693385TruSeq Adapter, Index 10 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGAGCTCGTATGC1394370.20050148882460547TruSeq Adapter, Index 10 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA1284230.18466406118406384No Hit
GATCGGAAGAGCACATGTCTGAACTCCAGTCACTCGAAGATCTCGTATGC1229700.1768229959104236TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACTCGAAGATCTCGTATGC1148800.1651900932763232TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACTCGAAGATCTCGTATGC1103160.15862735314999016TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACTCGAAGATCTCGTATGC1063920.15298489209483443TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGAAGATCTCGTATGC1058640.15222566186111317TruSeq Adapter, Index 19 (97% over 38bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA1055290.1517439532847938No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCATTCGAAGATCTCGTATGC1045810.15038078991061243TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACTCGAAGATCTCGTATGC973210.13994137419694508TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACTCGAAGATCTCGTATGC960580.13812526096741864TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACTCGAAGATCTCGTATGC915320.13161716241093677TruSeq Adapter, Index 3 (97% over 34bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATGCC866290.1245669619640895TruSeq Adapter, Index 10 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)