FastQCFastQC Report
Tue 26 Sep 2023
EGAF00004457780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95635353
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGC11421951.1943229822134918TruSeq Adapter, Index 7 (97% over 35bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA3924820.41039426079182245No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA3070970.32111242377073673No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT1439210.15048932793712802No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA1337820.1398875999338864No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)