FastQCFastQC Report
Mon 3 Apr 2023
EGAF00004457783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78118350
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTCGTATGC7689300.9843141848234122TruSeq Adapter, Index 9 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA2351660.3010381043634434No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA1967000.25179743299749674No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA919790.11774314229627227No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)