FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004457784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74015082
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGC12640791.707866783151034TruSeq Adapter, Index 7 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA2098260.28349087014454705No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA1638000.2213062467457646No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA831930.11240006462466665No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)