FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004460184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004460184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23986241
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG38200.023.2538848-49
CTCGTAT38950.022.20356844-45
CGTATGC40300.021.9259546-47
ATCTCGT53300.016.40167442-43
TGCCGTC55850.015.90507150-51
CCGTCTT69800.013.56802552-53
TCGTATG43050.013.53798846-47
ATGCCGT48100.012.31182650-51
TCTCGTA46650.012.09043644-45
GCCGTCT54950.011.0764152-53
AATCTCG59800.09.9817142-43
CGTCTGA106800.08.53713416-17
AGATCGG297650.07.83245994
ATCGGAA260750.07.552870894
CGTCTTC83050.07.272073354-55
TAGATCG101950.07.215217692-93
GTATGCC86050.07.15512848-49
GATCGGA272800.06.857424394
CACGTCT136800.06.83671814-15
TCGGAAG253800.06.83371994
Produced by FastQC (version 0.11.5)