FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004460187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004460187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20517142
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC323690.15776563811860345Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA47600.037.8693746-47
GCCGTAT52350.033.1318148-49
TCGCCGT53050.031.94107646-47
GTCGCCG56300.031.85027344-45
CCGTATC57150.031.54166448-49
CGTATCA59300.029.2887350-51
TGGTCGC61650.028.2475742-43
GGTCGCC63700.027.22788644-45
GTGGTCG68750.025.12526342-43
TCGGTGG83450.022.3872538-39
TCTCGGT84600.021.74878336-37
CGGTGGT86650.019.90746740-41
CTCGGTG94600.018.80462638-39
GATCTCG103550.018.58538434-35
AGAGCGT191250.018.528759
AAGAGCG211000.016.9726148
ATCTCGG114000.015.9543736-37
GTAGATC118300.015.03657332-33
GTATCAT130300.014.55592850-51
CGTGTAG122350.014.52010816-17
Produced by FastQC (version 0.11.5)