FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004671123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004671123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329168
Sequences flagged as poor quality0
Sequence length35-75
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGGGAGGGGAACAACACACACTGGGGCCTTTCGGGAGGCAGAGCGTTAAGAAAAACAGCTACTGCATGCTG14340.435643804987119No Hit
ACACAGGGAGGGGAACAACACACACTGGGGCCTTCCGGGAGACAGAGCGTTAAGAAAAACAGCTACTGCATGCTG9580.2910367958003208No Hit
ACACAGGGAGGGGAACAACACACACTGGGGCCTTTCGGGAGGCAGAGCGTTAAGAAAAACAGCTACCGCATGCTG6670.20263209060419No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4980.15129052641812082No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)