FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004671139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004671139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83134
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCAAGACAAACAAACTTGACCAAGTGACGCTTCTGGGTCAGTGTGCG6476777.90675295306373No Hit
GATCTGTATTTATTTCAGTGTTACTTACCTGTCTTGTCTTTGCTGATGTT48045.778622464936127No Hit
CAGCCAAGACAAACAAACTTGACCAAGTGATGCTTCTGGGTCAGTGTGCG33924.080159742103111No Hit
GATCTGTATTTATTTCAGTGTTACTTACCTGTCTTGTCTTTGTTGATGTT8110.9755334760747709No Hit
ACAGAGTAGAAGTGGTCAGCCTCAAAAGGCTGCCACTTGCAAAGTTTCTT2690.32357398898164413No Hit
CAGCCAAGACAACAAACTTGACCAAGTGACGCTTCTGGGTCAGTGTGCGT2500.30071932061491086No Hit
CAGCCAGACAAACAAACTTGACCAAGTGACGCTTCTGGGTCAGTGTGCGT2200.26463300214112156No Hit
CAGCCAAGACAAACAAACTAGACCAAGTGACGCTTCTGGGTCAGTGTGCG2040.24538696562176726No Hit
CAGCCAAGACAAACAACTTGACCAAGTGACGCTTCTGGGTCAGTGTGCGT2000.2405754564919287No Hit
CAGACAAGACAAACAAACTTGACCAAGTGACGCTTCTGGGTCAGTGTGCG1820.2189236654076551No Hit
CAGCCAAGACAAAAAAACTTGACCAAGTGACGCTTCTGGGTCAGTGTGCG1670.20088050617076045No Hit
CAGCCAAGACAAACAAACTTAACCAAGTGACGCTTCTGGGTCAGTGTGCG1640.19727187432338153No Hit
CACCCAAGACAAACAAACTTGACCAAGTGACGCTTCTGGGTCAGTGTGCG1610.19366324247600258No Hit
CAGCCAAGACAAACAAACTGGACCAAGTGACGCTTCTGGGTCAGTGTGCG1560.18764885606370438No Hit
CAGCAAAGACAAACAAACTTGACCAAGTGACGCTTCTGGGTCAGTGTGCG1540.1852431014987851No Hit
AAGCCAAGACAAACAAACTTGACCAAGTGACGCTTCTGGGTCAGTGTGCG1500.18043159236894651No Hit
CAGCCAAGACAAACAAACATGACCAAGTGACGCTTCTGGGTCAGTGTGCG1360.16359131041451153No Hit
CAGCAAGACAAACAAACTTGACCAAGTGACGCTTCTGGGTCAGTGTGCGT1360.16359131041451153No Hit
CAGCCAAGCAAACAAACTTGACCAAGTGACGCTTCTGGGTCAGTGTGCGT1340.1611855558495922No Hit
CAGCCAAGACAAACAAACGTGACCAAGTGACGCTTCTGGGTCAGTGTGCG1320.15877980128467295No Hit
CAGCCAAGACAAACAAACCTGACCAAGTGACGCTTCTGGGTCAGTGTGCG1240.14915678302499577No Hit
CAGCCAAGACAAACAAACTGACCAAGTGACGCTTCTGGGTCAGTGTGCGT1230.14795390574253614No Hit
CAGCCAACACAAACAAACTTGACCAAGTGACGCTTCTGGGTCAGTGTGCG1180.14193951933023793No Hit
CAGCCAAGACAAACAAACTTGACCAAGTGACGCTTCTGGGCCAGTGTGCG1080.1299107465056415No Hit
CAGCCAAACAAACAAACTTGACCAAGTGACGCTTCTGGGTCAGTGTGCGT1070.12870786922318186No Hit
CAGCCAAGACAAACAAACTTGACCAAGTGACGCTTCTGGGTCAGCGTGCG1060.1275049919407222No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1010.12149060552842399No Hit
CAGCCAAGACAAACAAACTTCACCAAGTGACGCTTCTGGGTCAGTGTGCG930.11186758726874685No Hit
CAGCCAAGACAAACAAACTTGACCAAGCGACGCTTCTGGGTCAGTGTGCG930.11186758726874685No Hit
CAGCCAAGACAAACAAAATTGACCAAGTGACGCTTCTGGGTCAGTGTGCG890.10705607813890827No Hit
CATCCAAGACAAACAAACTTGACCAAGTGACGCTTCTGGGTCAGTGTGCG880.10585320085644861No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)