FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004671154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004671154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences989264
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGATGGAGTTGGACATGGATACCTCCCAAGTCCTG104541.0567452166459106No Hit
TCCACAAAATGGATCCAGACACCATTTTCCTATCAGAGCAAGCA92610.9361505118957124No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC26290.26575312555596886TruSeq Adapter, Index 4 (100% over 50bp)
CTCCATCGCGCTCCTCAGACCTTGAGCTGTCCCTC19190.1939825971631435No Hit
AACTTCGTCCTCCAGAGTCGGAAGGCTCCATGCTGCTC17960.18154911125847092No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)