FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004671158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004671158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89835
Sequences flagged as poor quality0
Sequence length35-75
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGGGAGGGGAACAACACACACTGGGGCCTTTCGGGAGGCAGAGCGTTAAGAAAAACAGCTACTGCATGCTG5590.622251906272611No Hit
ACACAGGGAGGGGAACAACACACACTGGGGCCTTCCGGGAGACAGAGCGTTAAGAAAAACAGCTACTGCATGCTG3160.3517559971058051No Hit
ACACAGGGAGGGGAACAACACACACTGGGGCCTTTCGGGAGGCAGAGCGTTAAGAAAAACAGCTACCGCATGCTG2540.2827405799521345No Hit
ACACAGGGAGGGGAACAACACACACTGGGGCCTTTCGGGAGGCAGAGCGTTAAGAAAAACAGCTCCTGCATGCTG1090.12133355596371126No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)