FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004671165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004671165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119951
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAGACGACACAGGAAGCAGATTCTGCTAATACCCTGCAAATAGCAGAA5567146.41145134263158No Hit
TCAGCTCCATCCAAGTTCTGCACAGAGTAGAAGTGGTCAGCCTCAAAAGG4966441.40357312569299No Hit
ATCAGACGACACAGAAGCAGATTCTGCTAATACCCTGCAAATAGCAGAAA5890.49103383881751717No Hit
ATCAGACGACACGGAAGCAGATTCTGCTAATACCCTGCAAATAGCAGAAA3810.3176296987936741No Hit
ATCAGACGACACAGGAGCAGATTCTGCTAATACCCTGCAAATAGCAGAAA3420.2851164225392035No Hit
CAGCTCCATCCAAGTTCTGCACAGAGTAGAAGTGGTCAGCCTCAAAAGGC3360.28011438003851574No Hit
ACCAGACGACACAGGAAGCAGATTCTGCTAATACCCTGCAAATAGCAGAA3140.26177355753599385No Hit
ATAAGACGACACAGGAAGCAGATTCTGCTAATACCCTGCAAATAGCAGAA2780.23176130253186716No Hit
ATCAGACGACAAGGAAGCAGATTCTGCTAATACCCTGCAAATAGCAGAAA2480.20675109002842829No Hit
ATCAGACGACCAGGAAGCAGATTCTGCTAATACCCTGCAAATAGCAGAAA2370.19758067877716734No Hit
TCACTCCATCCAAGTTCTGCACAGAGTAGAAGTGGTCAGCCTCAAAAGGC2280.19007761502613568No Hit
TCAGCTCCATCAAAGTTCTGCACAGAGTAGAAGTGGTCAGCCTCAAAAGG1840.15339597002109195No Hit
ATCAGACGCACAGGAAGCAGATTCTGCTAATACCCTGCAAATAGCAGAAA1760.14672658002017488No Hit
TCAGCTCCATCAAGTTCTGCACAGAGTAGAAGTGGTCAGCCTCAAAAGGC1750.1458929062700603No Hit
TCAGCTCCATACAAGTTCTGCACAGAGTAGAAGTGGTCAGCCTCAAAAGG1730.144225558769831No Hit
TCAGCTCCATCCAGTTCTGCACAGAGTAGAAGTGGTCAGCCTCAAAAGGC1710.14255821126960175No Hit
ATCAGACGAACAGGAAGCAGATTCTGCTAATACCCTGCAAATAGCAGAAA1680.14005719001925787No Hit
ATCAGACCACACAGGAAGCAGATTCTGCTAATACCCTGCAAATAGCAGAA1590.13255412626822619No Hit
TCAGCTCCATCCAAGTTATGCACAGAGTAGAAGTGGTCAGCCTCAAAAGG1590.13255412626822619No Hit
TCAGCTCCATCCAAGTCCTGCACAGAGTAGAAGTGGTCAGCCTCAAAAGG1550.12921943126776766No Hit
TCAGATCCATCCAAGTTCTGCACAGAGTAGAAGTGGTCAGCCTCAAAAGG1530.1275520837675384No Hit
TCAGCTCCATCCAAGTTCTGCGTGACTCGAGTTCAGACTTATTGTGTAGAAGATACTCCAAT1510.12588473626730914No Hit
TCAGCTCATCCAAGTTCTGCACAGAGTAGAAGTGGTCAGCCTCAAAAGGC1460.12171636751673601No Hit
TCAGCTCCACCCAAGTTCTGCACAGAGTAGAAGTGGTCAGCCTCAAAAGG1390.11588065126593358No Hit
TCAGCCCATCCAAGTTCTGCACAGAGTAGAAGTGGTCAGCCTCAAAAGGC1370.11421330376570434No Hit
ATCAGACGACACAGGAAGCGGATTCTGCTAATACCCTGCAAATAGCAGAA1320.11004493501513117No Hit
TCAGCTCCATCCAATTCTGCACAGAGTAGAAGTGGTCAGCCTCAAAAGGC1290.10754391376478728No Hit
ACAGCTCCATCCAAGTTCTGCACAGAGTAGAAGTGGTCAGCCTCAAAAGG1280.10671024001467266No Hit
TAGCTCCATCCAAGTTCTGCACAGAGTAGAAGTGGTCAGCCTCAAAAGGC1270.10587656626455802No Hit
ATCAACGACACAGGAAGCAGATTCTGCTAATACCCTGCAAATAGCAGAAA1260.10504289251444339No Hit
TCAGCTACATCCAAGTTCTGCACAGAGTAGAAGTGGTCAGCCTCAAAAGG1200.10004085001375561No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)