FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004671166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004671166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65180
Sequences flagged as poor quality0
Sequence length35-75
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGGGAGGGGAACAACACACACTGGGGCCTTTCGGGAGGCAGAGCGTTAAGAAAAACAGCTACTGCATGCTG8721.3378336913163547No Hit
ACACAGGGAGGGGAACAACACACACTGGGGCCTTCCGGGAGACAGAGCGTTAAGAAAAACAGCTACTGCATGCTG5070.7778459650199447No Hit
ACACAGGGAGGGGAACAACACACACTGGGGCCTTTCGGGAGGCAGAGCGTTAAGAAAAACAGCTACCGCATGCTG1240.19024240564590364No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1020.15648972077324333No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)