FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004671167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004671167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72636
Sequences flagged as poor quality0
Sequence length35-75
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGGGAGGGGAACAACACACACTGGGGCCTTCCGGGAGACAGAGCGTTAAGAAAAACAGCTACTGCATGCTG6600.9086403436312572No Hit
ACACAGGGAGGGGAACAACACACACTGGGGCCTTTCGGGAGGCAGAGCGTTAAGAAAAACAGCTACTGCATGCTG4760.6553224296492097No Hit
ACACAGGGAGGGGAACAACACACACTGGGGCCTTTCGGGAGGCAGAGCGTTAAGAAAAACAGCTACCGCATGCTG1190.16383060741230243No Hit
ACACAGGGAGGGGAACATCACACACCGAGGCCTATCACGGGGTGGGGAACAAGGGGAGGGATAGCATTAGGAGA900.12390550140426236No Hit
ACACAGGGAGGGGAACATCACACACTGGGGCCTGTCGCAGGGTGGGGGTCTAGGGGAAGGATAGCATTAGGAGA880.12115204581750097No Hit
ACACAGGGAGGGGAACATCACACACCGGGGCCTTTCAGGGGGTAGAGGGCAAGGGGAGGAATAGCATTAGGAGA850.11702186243735889No Hit
ACACAGGGAGGGGAACAACACTTACTGGAGCCCGTCAGGAGAGGGTGGAGGAGGGGCAGAGCATTAGGGAAA770.10600804009031335No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)