FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004671168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004671168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94171
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCAGTGTTTTGAGTTCTAGTAGCACACACTCTGCACCTCGAAGGCT2979331.637128202949956No Hit
GAGGGTCCAGGTTCTTCCAGATGCTGATACTTTATTACATTTTGCCACAG1827319.404062821887845No Hit
TTTACCTCTATTGTTGGATCATATTCGTCCACAAAATGATTCTGAATTAG1655917.583969587240233No Hit
ACAGCCAGTGTTTTGAGTTCTAGTAGCACACACTCTGCACCTTGAAGGCT963910.235635174310563No Hit
GAGGGTCCAGGTTCTTCCAGATGCTGATACTTTATTACATTTAGTACTCC40484.298563251956547No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31333.326926548512812No Hit
TTTACCTCTATTGTTGGATCATATTCGTCCAGCCTGA6980.7412048295122703No Hit
ACAGCCAGTGTTTGAGTTCTAGTAGCACACACTCTGCACCTCGAAGGCTG4310.45767805375327864No Hit
GAGGGTCAAGGTTCTTCCAGATGCTGATACTTTATTACATTTTGCCACAG1360.14441813297087214No Hit
ACAGCCAGTGTTTGAGTTCTAGTAGCACACACTCTGCACCTTGAAGGCTG1350.1433562349343216No Hit
GAGGGTCCAGGTTCTCCCAGATGCTGATACTTTATTACATTTTGCCACAG1250.1327372545688163No Hit
GAGGGTCCACGTTCTTCCAGATGCTGATACTTTATTACATTTTGCCACAG1180.1253039683129626No Hit
GAGGGTCCAGGCTCTTCCAGATGCTGATACTTTATTACATTTTGCCACAG1090.11574688598400781No Hit
TGACAAAGAACAGCTCAAAGCAATTTCTACACGAGATCCTCTCTCTGAAA1090.11574688598400781No Hit
GAGGTCCAGGTTCTTCCAGATGCTGATACTTTATTACATTTTGCCACAGA1010.10725170169160357No Hit
GAGGGGCCAGGTTCTTCCAGATGCTGATACTTTATTACATTTTGCCACAG990.10512790561850252No Hit
TTACCTCTATTGTTGGATCATATTCGTCCACAAAATGATTCTGAATTAGC960.10194221150885091No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)