FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004671170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004671170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74062
Sequences flagged as poor quality0
Sequence length35-75
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGGGAGGGGAACAACACACACTGGGGCCTTCCGGGAGACAGAGCGTTAAGAAAAACAGCTACTGCATGCTG3650.4928303313440091No Hit
ACACAGGGAGGGGAACAGCACACACCAGGACCTGTTGGTGGGTGAGGGTTGATGAGAGAGAACTTAGAGAATAGG3560.4806783505711431No Hit
ACACAGGGAGGGGAACAACACACACTGGGGCCTTTCGGGAGGCAGAGCGTTAAGAAAAACAGCTACTGCATGCTG3280.4428721881666712No Hit
ACACAGGGAGGGGAACATCACACACCAGGGCAACCTAGATGACAGGTTGATGGGTGCAGCAAACCACCATGGCA3130.4226188868785612No Hit
ACACAGGGAGGGGAACAACACACACCAGGATGAGGGGAGGAAACTTAGAGGATGGGTTAATAGATGCAGCAAACC2860.3861629445599633No Hit
ACACAGGGAGGGGAACAACACACACCAGGATCTGTTTGGGGATGTGGAGAAAGGGGAGGGAACTTACAGGACA2490.3362048013826254No Hit
ACACAGGGAGGGGAACAACACACACCAGGGCCTGCTGAGGGGTGGAGGGTGAGAGGAGGGAACTTAGAGGATG2420.3267532607815074No Hit
ACACAGGGAGGGGAACATCATATAGATGATGGGTTGATGGGTGCAGCAAACCACCATGGCA2330.3146012800086414No Hit
ACACAGGGAGGGGAACATCACACAGGGCATATACCTAACACATGTGGAACTTAAAACCTAGATGACGGGTTCAT2150.29029731846290946No Hit
ACACAGGGAGGGGAACATCACCTACTGAGGACTGGATGGGTGCAGCAAACCACCATGGCA2040.2754448975182955No Hit
ACACAGGGAGGGGAACAACACACACCAGGGACTGTTGGGGGTAGTGGCCAAAGGATGGAAGCTTAAAGGATGGCT1950.26329291674542954No Hit
ACACAGGGAGGGGAACATCACACACCAGGGCCTATCAGGGAGTAGGGGGCTAGGGTTGATGGGTACAGCAAACC1950.26329291674542954No Hit
ACACAGGGAGGGGAACATCACATATCAGCGCCTGTCGCAAGGTGAGGGGCTAGGGGAGAGATAGCATTAAGAGA1870.2524911560584375No Hit
ACACAGGGAGGGGAACAACACACACCAGGGCCTGTTGCGGGTTAGAGGGTGAGGAGAGGGAACTCAGAGGACAGG1740.23493829494207558No Hit
ACACAGGGAGGGGAACAACATACCCTGGAGCCTGTTGAGCATTAGGAAAAATAGCTAATGCATGCTGGGCTTAA1730.23358807485620156No Hit
ACACAGGGAGGGGAACATCACACACTGGAGCCTGTTGGGGGATAGCATTAGGAGAAATACCTAATGTAAATGAC1660.22413653425508356No Hit
ACACAGGGAGGGGAACATCACACACCAGGGCCTGTTGAGGGATGAAGAACGAAGGGAGGGAACTTAGAAGATGGG1640.2214360940833356No Hit
ACACAGGGAGGGGAACATCACACACCAGGGCATGTCGAGAGGGATAGAATTAGGAGAAACACCTAATGTAGATG1630.22008587399746157No Hit
ACACAGGGAGGGGAACATCACATACTAGGACCTGTCAGGGGCTGAGGGGCATTAGGAGAAATACCTAATGT1610.2173854338257136No Hit
ACACAGGGAGGGGAACAAAACTCACTGGGCTTAATACCTGGGTGATGGGTTGATAGGTGCAGCAAACCACCATGG1550.2092841133104696No Hit
ACACAGGGAGGGGAACAACACATACCAGGGCCTGTTGGGAGGTTGGGGGTGAAGGTAGGGAACTCAGAGGATGGG1540.20793389322459563No Hit
ACACAGGGAGGGGAACATTAGGAGATAGCTGGGGGAGGGATAGCACTAGGAGAAATACCTAATGCAGATGATGGG1530.2065836731387216No Hit
ACACAGGGAGGGGAACATCATACACCAGGGCTAGGGGAGGGATAGCATTAGGAGAAATATCTAATGTAAATGAC1460.19713213253760362No Hit
ACACAGGGAGGGGAACAACACACACCAGAGCCTGTTGCGGGGTGAGGGGAGGGAACTTAGAGGATGGGTCAAT1430.19308147227998165No Hit
ACACAGGGAGGGGAACATCACACACTGGGGAACATCACACACTACTCCTACAAATACCTAATGTAGATGACAG1430.19308147227998165No Hit
ACACAGGGAGGGGAACAACACACATCAGAGCCTATTGGGAGGTGGGGAGTCAGGGGAGGGAACTTAGAGGATGGG1310.17687883124949366No Hit
ACACAGGGAGGGGAACATCACACACTGAGGTGAGGGGCTAAGGGATGGATAGCATTAGGAGAAACACTTAATATG1270.17147795090599768No Hit
ACACAGGGAGGGGAACATCACACACCGGGGCCTGTCAGGGAGTGGGGAGCTAGAGGAAGGATAGCATGGGTGC1260.1701277308201237No Hit
ACACAGGGAGGGGAACAACATACACCAAGGCGAGGGGAGGAAACTTAGAGGATGGGTTAACAGATGCAGCAAACC1240.1674272906483757No Hit
ACACAGGGAGGGGAACATCACACAGCAGGGCCTGTCCAGGGGTGCAGGCCTAGGGGAGGCATAGCATTAGGAGA1170.1579757500472577No Hit
ACACAGGGAGGGGAACATCACACACCAGGGCCTCTTGTGGGGAGGAAACTTGGAGGACAGGTCAATAGGTGCAGC1160.1566255299613837No Hit
ACACAGGGAGGGGAACATCGCACACTGGGGCCTGTTGTGGGATGGGTGGATAGGGGAGGGATAGTTGATGGGTGC1140.1539250897896357No Hit
ACACAGGGAGGGGAACAACGCACACTGAGACTGAGGAAAAATAGCTAATGCATGCTGGGCTTAATACCGAGGTG1130.1525748697037617No Hit
ACACAGGGAGGGGAACATCACACACTGGAGAGGGAGACCATTAGGACAAATAGCTAATGCATGCAGGGCTTAAA1100.14852420944613973No Hit
ACACAGGGAGGGGAACATCACACACCAGGGCCTCTTGTGGGGAGGAAACTTAGAGGACAGGTCAATAGGTGCAGC1090.14717398936026574No Hit
ACACAGGGAGGGGAACATCACACATTGGGGCCTTTTGGCAGGTAGGGAGCTAAGGGAGAGATAGCATTAGGAGA1040.14042288893089575No Hit
ACACAGGGAGGGGAACAACACATACCAGGGCCTGTCAGGGGTGAGGGACGAGGGGAGGGAACTTAGAGGATGGGT1020.13772244875914774No Hit
ACACAGGGAGGGGAACATCGCATTAGGAGAAATACCTAATGTAGATGACGGGTTGGTGGGTGCAGCAAACCACC990.13367178850152575No Hit
ACACAGGGAGGGGAACAACACACACTGGGGCCTTTCGGGAGGCAGAGCGTTAAGAAAAACAGCTACCGCATGCTG950.12827090815802974No Hit
ACACAGGGAGGGGAACATCACACACCAGGGCCTATTGAGGAGTGAGGGCTGAGGGGAGAGAACCTAGATGACAGG940.12692068807215576No Hit
ACACAGGGAGGGGAACATCACACACCAGGGCCTGTTGGAAGGTGGGGTGAGGAGAGGGAACGGACGGGTCAGT920.12422024790040777No Hit
ACACAGGGAGGGGAACAACACACACCAGGGCCTGGTGGGAGGGAACTTAGAGGACGGGTGCAGCAAACCACCATG920.12422024790040777No Hit
ACACAGGGAGGGGAACAACACACACCAGAGCCTGTTGGGGAGTCAGGGGCAAGGGGAAGGAACTTAGGGAATGGG910.12287002781453377No Hit
ACACAGGGAGGGGAACATCACACACCAGGGCCTGTCAGGGGATGGGGAGCTAGGAAGAAATACTAAATGTAGATG900.12151980772865976No Hit
ACACAGGGAGGGGAACATCACACACGGCCTGTCGGGGGATCAGGGGCTAGGAAAGGGATAGCATTAGGAGAAAT900.12151980772865976No Hit
ACACAGGGAGGGGAACACATAGGGCCTGTTGTGGGGTGGAAGGTGAGGGGAGTGAACCTAGAGGATGGGTCAATC890.12016958764278578No Hit
ACACAGGGAGGGGAACATCACACAATAGCATTAAGAGAAATACCTAATGTAAATGACTAGTTGATGGGTGCAGC880.11881936755691178No Hit
ACACAGGGAGGGGAACATCACACACTGGGGCCTGTCAAGGGGTGGGATAGCATTAGGAAAAATACCTAAAATGT850.11476870729928978No Hit
ACACAGGGAGGGGAACAACACACACCAGGGTCTGTTAGGAGGTGGGGATTGACAGGAGGGAACTTAAGGGATGGG850.11476870729928978No Hit
ACACAGGGAGGGGAACATCACACACCAGGGCCTAGTGGAGGGATAGCATTAGGAGAAATATCTAATGTATATGAT850.11476870729928978No Hit
ACACAGGGAGGGGAACAACATGCACCAGGGCATATTGGGGGGTGGAGGGTGAGTGGAGGGAACTTAGTGGACAGG850.11476870729928978No Hit
ACACAGGGAGGGGAACAACATACACCAAGGCGAGGGGAGGAAACTTAGAGGATGGGTTAACAGAAGCAGCAAACC840.1134184872134158No Hit
ACACAGGGAGGGGAACAACCCACACCAGGGCCTGTTGGGGGGTGGGGAGCAACAGGAGGGAACTTAGAGGATGGG830.11206826712754178No Hit
ACACAGGGAGGGGAACATCATACACTGGGGCTTGTTTGGGGGTGACAGGTTGATGGGTGCAGCAAACCACCATGG820.1107180470416678No Hit
ACACAGGGAGGGGAACATCACATACCAGTCAGGGGAGGGATAGCACTAGGAGAAATACCTAATGTAGGTGACAGG810.10936782695579379No Hit
ACACAGGGAGGGGAACATCACACACCAGGTCCTGTCAGGGGAGTGGAAGTCAAGGGGAGGGATAGCATTAGA810.10936782695579379No Hit
ACACAGGGAGGGGAACATCACACACTGGAGCCGGTGGGCGGGGAAGGGTGAGGGTGATGGGTTGATAGGTGCAGC810.10936782695579379No Hit
ACACAGGGAGGGGAACAACACACACCAGGGCCTGTTTGGAGGTGGGAGGTGAGGGGAGGGAATTTAGAGGACAGG800.1080176068699198No Hit
ACACAGGGAGGGGAACAACGCACACCAGGGCATGTTGCAGGGTGAGGGGTGAGGGGAGGGAATTCAGAGGATGGG800.1080176068699198No Hit
ACACAGGGAGGGGAACATCACACACCAGGGTCTGTCAGGGGATGGAGATAGCATTAGAAGAAATACCTAATGT790.10666738678404579No Hit
ACACAGGGAGGGGAACAACACACACCAGGGCCTGTTGGTCAGGGGGTGGTGAGGGGAAGGAACCTAGATGATGGG780.1053171666981718No Hit
ACACAGGGAGGGGAACAACACACACCATGGCCTGTTGGGAAGTTGGGGATGAGGGGAAGGAACTTAGAGGATGGG750.10126650644054981No Hit
ACACAGGGAGGGGAACATCACACACTGGGGCCTCTTGGGGGGATAGCATTATGAGAAATACCTAATGTAGATGAT750.10126650644054981No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)