FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004671173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004671173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128538
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATGGTGTCGCAGAAGCAGGCGCAGTCCCCAATGGACTCACAGGAGCAG5299041.22516298682102No Hit
TTTACCTCTATTGTTGGATCATATTCGTCCACAAAATGATTCTGAATTAG5294541.1901538844544No Hit
CAATGGTGTCGCAGAAGCAGGCGCAGTCCCCAGTGGACTCACAGGAGCAG49233.8299957989077162No Hit
CAATGGTGTCGCAAAGCAGGCGCAGTCCCCAATGGACTCACAGGAGCAGG4040.31430394124694644No Hit
CAATGGTGTCCAGAAGCAGGCGCAGTCCCCAATGGACTCACAGGAGCAGG3690.2870746394062456No Hit
CAATGGTGCCGCAGAAGCAGGCGCAGTCCCCAATGGACTCACAGGAGCAG3640.28318473914328834No Hit
CAATGTGTCGCAGAAGCAGGCGCAGTCCCCAATGGACTCACAGGAGCAGG3410.265291197933685No Hit
CAATGGTGTCGCAGAGCAGGCGCAGTCCCCAATGGACTCACAGGAGCAGG3410.265291197933685No Hit
CAATGGTGCGCAGAAGCAGGCGCAGTCCCCAATGGACTCACAGGAGCAGG3220.2505095769344474No Hit
TTACCTCTATTGTTGGATCATATTCGTCCACAAAATGATTCTGAATTAGC3050.23728391604039273No Hit
CAATGGTGTGCAGAAGCAGGCGCAGTCCCCAATGGACTCACAGGAGCAGG2800.2178344147256064No Hit
CAATGGTGTCGAGAAGCAGGCGCAGTCCCCAATGGACTCACAGGAGCAGG2760.21472249451524061No Hit
CAAGGGTGTCGCAGAAGCAGGCGCAGTCCCCAATGGACTCACAGGAGCAG2600.2022748136737774No Hit
TCAGCTCCATCCAAGTTCTGCACAGAGTAGAAGTGGTCAGCCTCAAAAGG2480.19293905304268No Hit
CAATGGCGTCGCAGAAGCAGGCGCAGTCCCCAATGGACTCACAGGAGCAG2370.18438127246417402No Hit
CAATGGTTCGCAGAAGCAGGCGCAGTCCCCAATGGACTCACAGGAGCAGG2100.16337581104420482No Hit
TTTACCTCTATTGTTGGATAATATTCGTCCACAAAATGATTCTGAATTAG2010.15637399057088175No Hit
TTTACCTCTATCGTTGGATCATATTCGTCCACAAAATGATTCTGAATTAG1910.14859419004496724No Hit
CATGGTGTCGCAGAAGCAGGCGCAGTCCCCAATGGACTCACAGGAGCAGG1770.13770246930868693No Hit
TTTACCTCTATTGTTGGATCATAGTCGTCCACAAAATGATTCTGAATTAG1690.1314786288879553No Hit
CAATGGGTCGCAGAAGCAGGCGCAGTCCCCAATGGACTCACAGGAGCAGG1610.1252547884672237No Hit
CAATGGGGTCGCAGAAGCAGGCGCAGTCCCCAATGGACTCACAGGAGCAG1520.11825296799390064No Hit
TTTACCTCTATTGTTGGATCATATTCGCCCACAAAATGATTCTGAATTAG1460.11358508767835193No Hit
TTTACCTCTATGTTGGATCATATTCGTCCACAAAATGATTCTGAATTAGC1430.11125114752057758No Hit
TTTACCTCTATTGTTGGATCATATCGTCCACAAAATGATTCTGAATTAGC1430.11125114752057758No Hit
TTTACCTCTATTGTTGGAGCATATTCGTCCACAAAATGATTCTGAATTAG1350.10502730709984595No Hit
TTTACCTCTATTGTTGGATCATATTAGTCCACAAAATGATTCTGAATTAG1330.10347134699466305No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)