FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004671174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004671174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66855
Sequences flagged as poor quality0
Sequence length35-75
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25463.8082417171490537No Hit
ACACAGGGAGGGGAACAACACACACTGGGGCCTTTCGGGAGGCAGAGCGTTAAGAAAAACAGCTACTGCATGCTG7031.051529429362052No Hit
ACACAGGGAGGGGAACAACACACACTGGGGCCTTCCGGGAGACAGAGCGTTAAGAAAAACAGCTACTGCATGCTG5570.8331463615286815No Hit
ACACAGGGAGGGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATGCCGTCTTCTGCTTGAA1060.15855209034477602TruSeq Adapter, Index 7 (96% over 32bp)
ACACAGGGAGGGGAACAACACACACTGGGGCCTGTCAGGGAGATCATCAGGATAAATAGCTAATGCATGAGGGGC960.14359434597262732No Hit
ACACAGGGAGGGGAACAACACACACTGGGGCCTTTCGGGAGGCAGAGCGTTAAGAAAAACAGCTACCGCATGCTG870.1301323760376935No Hit
ACACAGGGAGGGGAACATCACACACCAGGGCAACCTAGATGACAGGTTGATGGGTGCAGCAAACCACCATGGCA710.10619998504225563No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)