FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004671176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004671176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395908
Sequences flagged as poor quality0
Sequence length35-75
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGGGAGGGGAACAACACACACTGGGGCCTTCCGGGAGACAGAGCGTTAAGAAAAACAGCTACTGCATGCTG25520.6445941986522121No Hit
ACACAGGGAGGGGAACAACACACACTGGGGCCTTTCGGGAGGCAGAGCGTTAAGAAAAACAGCTACTGCATGCTG14140.3571536821685846No Hit
ACACAGGGAGGGGAACAACACACACTGGGGCCTTTCGGGAGGCAGAGCGTTAAGAAAAACAGCTACCGCATGCTG5220.13184881336067975No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)