FastQCFastQC Report
Sat 11 May 2024
EGAF00004671609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004671609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28294664
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTAACGAATCTCGTAT1803010.6372261568471002TruSeq Adapter, Index 6 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTAACGAATCGCGTAT620580.21932757356652124TruSeq Adapter, Index 6 (97% over 38bp)
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG432240.15276378613296132No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC373090.13185878439835866No Hit
CCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCAT357900.12649028099432458No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG339340.11993074029788799No Hit
GTCCGCTCCGGGCCGGGACGGGGTCCGGGGAGCGTGGTTTGGGAGCCGCG312690.11051200325262742No Hit
GTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTT286890.10139367620693428No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)