FastQCFastQC Report
Sat 11 May 2024
EGAF00004671611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004671611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25803145
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGAGTAAATCTCGTAT597560.23158417316958843TruSeq Adapter, Index 6 (97% over 37bp)
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG406470.15752730917103322No Hit
CCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCAT386630.1498383239717484No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG325120.12600014455602215No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC312260.121016255964147No Hit
GTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTT272960.10578555443532174No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)