FastQCFastQC Report
Mon 13 May 2024
EGAF00004671837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004671837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98367377
Total Bases9.9 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTGGCCTTCGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT59115006.009614346024496No Hit
AGCTGGCCTTCTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA15546371.5804396207494686No Hit
AGCTGGCCTTCGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC3698270.3759650925733234No Hit
GCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCA3508060.3566283972378363No Hit
AAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTT2542740.25849423635642943No Hit
AACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTG2485910.2527169144705363No Hit
ACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGC2297460.23355914024219634No Hit
CTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCT1916000.1947800234624534No Hit
GAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAGAT1856350.18871602116624497No Hit
GACCAATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCATA1771120.18005156323320484No Hit
GCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAA1705950.1734263992827622No Hit
GGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGG1678360.1706216076087909No Hit
GCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAAAGGC1569060.15951020021607365No Hit
GCCAGTTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGCC1564040.15899986842182443No Hit
CAAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCT1454520.14786609589071384No Hit
CGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAGA1410580.14339916779523357No Hit
TGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTG1356840.13793597444404765No Hit
CCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAG1317360.1339224486996334No Hit
AGCTGGCCTTCGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC1301610.13232130811010645No Hit
CGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTC1298440.13199904679780167No Hit
CAGTTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGCCCC1215230.12353994149910087No Hit
GTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGT1140990.11599272388853066No Hit
CTGGCGCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAG1133270.11520791085036253No Hit
AGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAGATT1131150.11499239224402619No Hit
AAGACGACCAATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCG1097600.11158170863903386No Hit
CGCCAGTTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGC1022180.10391453255889907No Hit
AATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCATACGCT1011510.10282982334681955No Hit
CTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCATACGCTCGG988730.10051401492590373No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)