FastQCFastQC Report
Wed 22 Mar 2023
EGAF00004671858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004671858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89176026
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTTCAAGAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT50758185.691908719951257No Hit
TCACTTCAAGATGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA17254741.9349079314209403No Hit
TCACTTCAAGAGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC4692840.5262445760926822No Hit
GCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCA3760200.4216604135286316No Hit
GAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAGAT2674070.2998642258402499No Hit
TGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTG2319930.26015175872492907No Hit
AAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTT2193820.24601006553039267No Hit
ACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGC2164200.24268854501320794No Hit
AACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTG2101360.23564180803481868No Hit
GGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGG2039150.2286657178466329No Hit
CGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAGA1913610.2145879431765663No Hit
CTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCT1876940.2104758514356762No Hit
GCCAGTTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGCC1607070.18021323354328436No Hit
CAGTTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGCCCC1521740.17064451829239397No Hit
CGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTC1450600.16266703788751474No Hit
GCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAA1429340.16028298906255364No Hit
GTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGT1354390.15187826378358685No Hit
GCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAAAGGC1338180.1500605106578757No Hit
CCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAG1333340.1495177638886936No Hit
CCAGTTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGCCC1274090.14287360147670183No Hit
CAAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCT1202030.1347929543305731No Hit
CGCCAGTTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGC1195880.13410330709287271No Hit
AGTTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGCCCCT1175300.13179551194622646No Hit
GGCGCCAGTTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAA1089680.12219427674429No Hit
TTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGCCCCTGC1082380.12137567108002772No Hit
AGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAGATT1068940.11986853955568731No Hit
GTAAAGGCGCTCGTCTTTGGTATGTAGGTGGTCAACAATTTTAATTGCAG1015470.1138725334093717No Hit
AGGGGCTTCGGCCCCTTACTTGAGGATAAATTATGTCTAATATTCAAACT978380.10971334380834598No Hit
GAGGTACTAAAGGCAAGCGTAAAGGCGCTCGTCTTTGGTATGTAGGTGGT959170.10755917739595168No Hit
GCTCGTCTTTGGTATGTAGGTGGTCAACAATTTTAATTGCAGGGGCTTCG952400.1068000047456701No Hit
GTTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGCCCCTG934740.10481965186472876No Hit
GCTCGGCGCCAGTTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGC924450.10366575429140562No Hit
TGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGCCCCTGCAA915710.10268567024953544No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)