FastQCFastQC Report
Thu 23 Mar 2023
EGAF00004671859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004671859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89176026
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTTCAAGAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT52392875.875219198487271No Hit
CACTTCAAGAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGTA16926401.8980886185710946No Hit
TCACTTCAAGATGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA16217541.8185986444383608No Hit
TCACTTCAAGAGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTC4123560.46240679081169195No Hit
CACTTCAAGATGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAAC3428610.38447665295154554No Hit
CTTCAAGAGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGTAGT2643200.29640253312028053No Hit
GCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCA2519210.28249857198166695No Hit
GCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAA1717280.1925719363184002No Hit
GCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAAAGGC1624940.18221713535429354No Hit
GACCAATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCATA1558730.1747924941171969No Hit
AAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTT1459270.16363927228602898No Hit
GCCAGTTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGCC1452670.16289916305532612No Hit
GAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAGAT1429180.1602650470205972No Hit
CTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCT1420390.15927935609061564No Hit
ACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGC1375870.15428698291623805No Hit
GGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGG1276840.14318198032282803No Hit
AACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTG1254650.14069364337899515No Hit
CTGGCGCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAG1204200.13503629327460723No Hit
CAGTTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGCCCC1149180.12886647359683867No Hit
CGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAGA1058290.1186742723879622No Hit
CAAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCT1054760.11827842608729838No Hit
GTTCTGGCGCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGC1020110.11439285262610827No Hit
CCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTGGTCAG987720.11076071050755278No Hit
TGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTTGCTG977650.10963148324191975No Hit
CGCCAGTTTGAATATTAGACATAATTTATCCTCAAGTAAGGGGCCGAAGC966270.10835535550776842No Hit
CTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAAA927420.10399880344522192No Hit
CACTTCAAGAGTACGCTGGACTTTGTAGGATACCCTCGCTTTCCTGCTCC912320.10230552323558352No Hit
AATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCGGCATACGCT908510.10187827836149596No Hit
AAGACGACCAATCTGACCAGCAAGGAAGCCAAGATGGGAAAGGTCATGCG905430.1015328940538346No Hit
GTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAAAGGCGCTCGTCTT900480.10097781213080745No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)