FastQCFastQC Report
Wed 11 May 2022
EGAF00004675319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52739274
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA14746992.7962064855121063TruSeq Adapter, Index 19 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2082000.3947722147255952TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1169640.22177779694123206TruSeq Adapter, Index 19 (97% over 40bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTGAGATC739540.1402256693939321No Hit
TGGGTGTGGTGGTGGGTGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGTGTGAATTTGAGATC665770.12623799106525432No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA649370.12312835402322755TruSeq Adapter, Index 19 (97% over 40bp)
CGGGAAGCGGAGTTTGTAGTGAGTCGAGATTGCGTTATTGTAGTTCGTAGTTTGAGATCGGAAGAGCACACGTCT607600.11520826016679714Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGATTGCGGATTGTAGTGGCGTAATTTCGGTTTATTGTAAGTTTCGTTTTTTGAGATCGGAAGAGCACACGTCT545280.10339163940709535Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGTGTAGTGGTGGGTGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGTGTGAATTTGAGATC532030.10087928021155544No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)