FastQCFastQC Report
Thu 12 May 2022
EGAF00004675320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52739274
Sequences flagged as poor quality0
Sequence length75
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA663520.12581136403204943Illumina Single End PCR Primer 1 (100% over 57bp)
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAAACGCCCGCCACTACGCCCGAGATC663500.12580757179175428No Hit
CAAATTCACACCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAAACACCCACCACCACACCCAAGATC618470.11726934276721367No Hit
CAAACTACGAACTACAATAACGCAATCTCGACTCACTACAAACTCCGCTTCCCGAGATCGGAAGAGCGTCGTGTA574180.10887142663359378Illumina Single End PCR Primer 1 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)