FastQCFastQC Report
Sun 5 Jun 2022
EGAF00004675321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62504131
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA22407003.5848830535696914TruSeq Adapter, Index 7 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA4885810.7816779342152601TruSeq Adapter, Index 7 (97% over 44bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTGAGATC982330.15716241219320368No Hit
CGGGAAGCGGAGTTTGTAGTGAGTCGAGATTGCGTTATTGTAGTTCGTAGTTTGAGATCGGAAGAGCACACGTCT836900.13389515006616123Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGATTGCGGATTGTAGTGGCGTAATTTCGGTTTATTGTAAGTTTCGTTTTTTGAGATCGGAAGAGCACACGTCT743040.11887854260384804Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)