FastQCFastQC Report
Thu 12 May 2022
EGAF00004675323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60222298
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA17575872.9184987261694992TruSeq Adapter, Index 8 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1846300.3065807950404018TruSeq Adapter, Index 8 (97% over 44bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTGAGATC884830.14692730589589922No Hit
CGGGAAGCGGAGTTTGTAGTGAGTCGAGATTGCGTTATTGTAGTTCGTAGTTTGAGATCGGAAGAGCACACGTCT672080.11159985957360843Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGATTGCGGATTGTAGTGGCGTAATTTCGGTTTATTGTAAGTTTCGTTTTTTGAGATCGGAAGAGCACACGTCT616830.10242551687416511Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)