FastQCFastQC Report
Sat 4 Jun 2022
EGAF00004675325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79049347
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA18133982.294007564667169TruSeq Adapter, Index 11 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA3487440.44117252480276653TruSeq Adapter, Index 11 (97% over 44bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTGAGATC1455330.1841039876015674No Hit
CGGGAAGCGGAGTTTGTAGTGAGTCGAGATTGCGTTATTGTAGTTCGTAGTTTGAGATCGGAAGAGCACACGTCT1388200.1756118238396074Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGATTGCGGATTGTAGTGGCGTAATTTCGGTTTATTGTAAGTTTCGTTTTTTGAGATCGGAAGAGCACACGTCT1197820.1515281334329049Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGATGGTTTCGATTTTTTGATTTCGTGATTCGTTCGTTTCGGTTTTTTAAAGTGTTGGGATTATAGGCGTGAG860100.10880545287742859No Hit
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTGAGATC824050.10424501039837811No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)