FastQCFastQC Report
Thu 12 May 2022
EGAF00004675327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61453332
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA22091643.5948644737440762TruSeq Adapter, Index 12 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2173490.35368139192192216TruSeq Adapter, Index 12 (97% over 44bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA985040.16029073899524277TruSeq Adapter, Index 12 (97% over 43bp)
CGGGAAGCGGAGTTTGTAGTGAGTCGAGATTGCGTTATTGTAGTTCGTAGTTTGAGATCGGAAGAGCACACGTCT857360.1395139973858537Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTGAGATC829690.13501139368651321No Hit
CGGATTGCGGATTGTAGTGGCGTAATTTCGGTTTATTGTAAGTTTCGTTTTTTGAGATCGGAAGAGCACACGTCT743740.12102517077511761Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA614830.10004827728462307TruSeq Adapter, Index 12 (97% over 41bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)