FastQCFastQC Report
Thu 12 May 2022
EGAF00004675329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61804658
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA24412443.949935294521005TruSeq Adapter, Index 10 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA3059920.49509536967262235TruSeq Adapter, Index 10 (97% over 44bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTGAGATC800190.12947082402753526No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA655190.10600980916357469TruSeq Adapter, Index 10 (97% over 41bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)