FastQCFastQC Report
Wed 11 May 2022
EGAF00004675331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55666213
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA20457983.675116178641432TruSeq Adapter, Index 9 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2622210.47105952761686876TruSeq Adapter, Index 9 (97% over 44bp)
CGGGAAGCGGAGTTTGTAGTGAGTCGAGATTGCGTTATTGTAGTTCGTAGTTTGAGATCGGAAGAGCACACGTCT724170.13009147936828394Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTGAGATC679700.12210279150837869No Hit
CGGATTGCGGATTGTAGTGGCGTAATTTCGGTTTATTGTAAGTTTCGTTTTTTGAGATCGGAAGAGCACACGTCT642930.11549734845443861Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA600310.10784099863233017TruSeq Adapter, Index 9 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA582240.10459486439287687TruSeq Adapter, Index 9 (97% over 41bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)