FastQCFastQC Report
Sun 5 Jun 2022
EGAF00004675332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55666213
Sequences flagged as poor quality0
Sequence length75
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA1046880.18806380811283138Illumina Single End PCR Primer 1 (100% over 57bp)
CAAACTACGAACTACAATAACGCAATCTCGACTCACTACAAACTCCGCTTCCCGAGATCGGAAGAGCGTCGTGTA693510.12458365004998632Illumina Single End PCR Primer 1 (100% over 21bp)
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAAACGCCCGCCACTACGCCCGAGATC644440.11576860815015384No Hit
CAAAAAACGAAACTTACAATAAACCGAAATTACGCCACTACAATCCGCAATCCGAGATCGGAAGAGCGTCGTGTA621370.11162426299773616Illumina Single End PCR Primer 1 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)