FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004675333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50177115
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA19439523.874180490448684TruSeq Adapter, Index 8 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1717850.3423572678500946TruSeq Adapter, Index 8 (97% over 44bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTGAGATC624920.12454283192646688No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA603580.12028989709751148TruSeq Adapter, Index 8 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA591640.11791032625131995TruSeq Adapter, Index 8 (97% over 41bp)
CGGGAAGCGGAGTTTGTAGTGAGTCGAGATTGCGTTATTGTAGTTCGTAGTTTGAGATCGGAAGAGCACACGTCT587490.11708325598233378Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA549570.10952602595824809TruSeq Adapter, Index 8 (97% over 41bp)
CGGATTGCGGATTGTAGTGGCGTAATTTCGGTTTATTGTAAGTTTCGTTTTTTGAGATCGGAAGAGCACACGTCT549300.10947221656725382Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)