FastQCFastQC Report
Sun 5 Jun 2022
EGAF00004675335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54629384
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA17312383.169060079461998TruSeq Adapter, Index 7 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1455740.2664756388247028TruSeq Adapter, Index 7 (97% over 44bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTGAGATC893900.16362988826672475No Hit
CGGGAAGCGGAGTTTGTAGTGAGTCGAGATTGCGTTATTGTAGTTCGTAGTTTGAGATCGGAAGAGCACACGTCT764600.1399613072700948Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGATTGCGGATTGTAGTGGCGTAATTTCGGTTTATTGTAAGTTTCGTTTTTTGAGATCGGAAGAGCACACGTCT655600.1200086751847687Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGATGGTTTCGATTTTTTGATTTCGTGATTCGTTCGTTTCGGTTTTTTAAAGTGTTGGGATTATAGGCGTGAG620560.11359454465018314No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)