FastQCFastQC Report
Thu 12 May 2022
EGAF00004675336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54629384
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAAACGCCCGCCACTACGCCCGAGATC856070.15670504357142304No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA799590.14636628522115497Illumina Single End PCR Primer 1 (100% over 57bp)
CAAACTACGAACTACAATAACGCAATCTCGACTCACTACAAACTCCGCTTCCCGAGATCGGAAGAGCGTCGTGTA743460.13609159495556458Illumina Single End PCR Primer 1 (100% over 21bp)
CAAACGCGATAACTCACGCCTATAATCCCAACACTTTAAAAAACCGAAACGAACGAATCACGAAATCAAAAAATC717630.1313633703063538No Hit
CAAAAAACGAAACTTACAATAAACCGAAATTACGCCACTACAATCCGCAATCCGAGATCGGAAGAGCGTCGTGTA645350.118132395562066Illumina Single End PCR Primer 1 (100% over 21bp)
CAAAATAATCTCGATCTCCTAACCTCGTAATCCGCCCGCCTCGACCTCCCAAAATACTAAAATTACAAACGTAAA559410.1024009349986447No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)