FastQCFastQC Report
Thu 12 May 2022
EGAF00004675338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53912442
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAAACGCCCGCCACTACGCCCGAGATC1240480.23009159926385822No Hit
CAAACTACGAACTACAATAACGCAATCTCGACTCACTACAAACTCCGCTTCCCGAGATCGGAAGAGCGTCGTGTA1033800.19175536511590402Illumina Single End PCR Primer 1 (100% over 21bp)
CAAACGCGATAACTCACGCCTATAATCCCAACACTTTAAAAAACCGAAACGAACGAATCACGAAATCAAAAAATC966250.17922578984643286No Hit
CAAAAAACGAAACTTACAATAAACCGAAATTACGCCACTACAATCCGCAATCCGAGATCGGAAGAGCGTCGTGTA927700.1720753068466088Illumina Single End PCR Primer 1 (100% over 21bp)
CAAAATAATCTCGATCTCCTAACCTCGTAATCCGCCCGCCTCGACCTCCCAAAATACTAAAATTACAAACGTAAA801680.14870036864588698No Hit
CAAATTCACGCCATTCTCCTACCTCAACCTCCCGAATAACTAAAACTACAAACGCCCGCCACCGCGCCCGAGATC684750.12701149764278902No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA650810.12071610482789855Illumina Single End PCR Primer 1 (100% over 57bp)
CAAACGTAATAACGAACGCCTATAATCCCAACTACTTAAAAAACTAAAACAAAAAAATAACGTAAACCCGAGATC603620.11196302330360032No Hit
CAAACGTAATAACGAACGCCTATAATCCCAACTACTCGAAAAACTAAAACAAAAAAATAACGTAAACCCGAGATC591230.10966485250287865No Hit
CAAACGCGATAACGAACGCCTATAATCCCAACTACTCGAAAAACTAAAACAAAAAAATAACGTAAACCCGAGATC544380.10097483619829352No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)