FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004675339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46681613
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA18816724.030863286579237TruSeq Adapter, Index 9 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2255690.48320738188716833TruSeq Adapter, Index 9 (97% over 44bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTGAGATC1089190.2333231287445016No Hit
CGGGAAGCGGAGTTTGTAGTGAGTCGAGATTGCGTTATTGTAGTTCGTAGTTTGAGATCGGAAGAGCACACGTCT878270.18814045692894116Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGATTGCGGATTGTAGTGGCGTAATTTCGGTTTATTGTAAGTTTCGTTTTTTGAGATCGGAAGAGCACACGTCT787570.16871096549298759Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGATGGTTTCGATTTTTTGATTTCGTGATTCGTTCGTTTCGGTTTTTTAAAGTGTTGGGATTATAGGCGTGAG685280.1467986978084926No Hit
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTGAGATC610160.13070670887057823No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA601670.12888800564796252TruSeq Adapter, Index 9 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA560500.12006868742945964TruSeq Adapter, Index 9 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA554430.1187683896012762TruSeq Adapter, Index 9 (97% over 41bp)
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGCGGGCGGATTACGAGGTTAGGAGATC505480.10828246230480511No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATAGGCGTTCGTTATTACGTTTGAGATC490780.10513347085928672No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATAGGCGTTCGTTATTACGTTTGAGATC468720.10040784151995777No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTAA208.189966E-569.5489041
CCGGTAC800.069.5489041
CGCAACG208.189966E-569.5489041
ACACTGG150.002283252569.54891
GCCTAGT150.002285483869.531784
CAAGCGA401.546141E-1068.949827
CTATACC208.54872E-568.9493847
CGCCTAC401.546141E-1068.9493853
ACTACTG208.54872E-568.9493855
CCCTATC401.546141E-1068.9486436
ACCCGAT150.002363099568.9486435
CCGGCGC2950.068.370111
CCGTTAA2950.067.1913151
CCGGCGT8050.065.229091
CCGGATG1600.065.2020951
CGCTTAA2000.064.332731
CGGGCCT600.063.753161
CGGTTAA573500.063.752151
CCGGTAT3500.063.5875661
CCGGTTT9900.063.2262761
Produced by FastQC (version 0.11.5)