FastQCFastQC Report
Sat 4 Jun 2022
EGAF00004675341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69469974
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA23918653.44301985775898TruSeq Adapter, Index 11 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA3601160.5183764715386248TruSeq Adapter, Index 11 (97% over 44bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTGAGATC1269180.1826947567304401No Hit
CGGGAAGCGGAGTTTGTAGTGAGTCGAGATTGCGTTATTGTAGTTCGTAGTTTGAGATCGGAAGAGCACACGTCT1078610.1552627614341701Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGATTGCGGATTGTAGTGGCGTAATTTCGGTTTATTGTAAGTTTCGTTTTTTGAGATCGGAAGAGCACACGTCT945370.1360832523127186Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGTGTGGTGGTGGGTGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGTGTGAATTTGAGATC841470.12112715055859961No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA808880.11643591517682156TruSeq Adapter, Index 11 (97% over 41bp)
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTGAGATC704970.10147837395188891No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)