FastQCFastQC Report
Sat 4 Jun 2022
EGAF00004675342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69469974
Sequences flagged as poor quality0
Sequence length75
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAAACGCCCGCCACTACGCCCGAGATC1137040.16367358939849322No Hit
CAAACTACGAACTACAATAACGCAATCTCGACTCACTACAAACTCCGCTTCCCGAGATCGGAAGAGCGTCGTGTA1010580.1454700414887157Illumina Single End PCR Primer 1 (100% over 21bp)
CAAAAAACGAAACTTACAATAAACCGAAATTACGCCACTACAATCCGCAATCCGAGATCGGAAGAGCGTCGTGTA894510.12876210375435004Illumina Single End PCR Primer 1 (100% over 21bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA851450.1225637424306507Illumina Single End PCR Primer 1 (100% over 57bp)
CAAATTCACACCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAAACACCCACCACCACACCCAAGATC772180.11115305729062169No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)