FastQCFastQC Report
Sun 5 Jun 2022
EGAF00004675343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54779690
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA18572233.390349598546469TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2061160.3762635385486848TruSeq Adapter, Index 13 (97% over 40bp)
CGGGAAGCGGAGTTTGTAGTGAGTCGAGATTGCGTTATTGTAGTTCGTAGTTTGAGATCGGAAGAGCACACGTCT923510.16858620412054176Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTGAGATC886370.16180631909381013No Hit
TGGGTGTGGTGGTGGGTGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGTGTGAATTTGAGATC782090.14277006678935203No Hit
CGGATTGCGGATTGTAGTGGCGTAATTTCGGTTTATTGTAAGTTTCGTTTTTTGAGATCGGAAGAGCACACGTCT764330.1395279892967631Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGTGTAGTGGTGGGTGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGTGTGAATTTGAGATC732820.13377585743913484No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA578430.10559205428143169TruSeq Adapter, Index 13 (97% over 40bp)
CGGGATGGTTTCGATTTTTTGATTTCGTGATTCGTTCGTTTCGGTTTTTTAAAGTGTTGGGATTATAGGCGTGAG572290.1044712009140614No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)