FastQCFastQC Report
Thu 12 May 2022
EGAF00004675344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54779690
Sequences flagged as poor quality0
Sequence length75
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAACTACGAACTACAATAACGCAATCTCGACTCACTACAAACTCCGCTTCCCGAGATCGGAAGAGCGTCGTGTA878690.16040433963755543Illumina Single End PCR Primer 1 (100% over 21bp)
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAAACGCCCGCCACTACGCCCGAGATC810090.1478814502236139No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA748610.1366583125972418Illumina Single End PCR Primer 1 (100% over 57bp)
CAAAAAACGAAACTTACAATAAACCGAAATTACGCCACTACAATCCGCAATCCGAGATCGGAAGAGCGTCGTGTA733950.13398213827058897Illumina Single End PCR Primer 1 (100% over 21bp)
CAAATTCACACCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAAACACCCACCACCACACCCAAGATC728690.1330219283825812No Hit
CAAATTCACACCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAAACACCCACCACTACACCCAAGATC678940.12394009531634807No Hit
CAAACGCGATAACTCACGCCTATAATCCCAACACTTTAAAAAACCGAAACGAACGAATCACGAAATCAAAAAATC563850.10293048390744818No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)