FastQCFastQC Report
Thu 12 May 2022
EGAF00004675345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58193387
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA16351182.8098003644297247TruSeq Adapter, Index 14 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2026320.348204513341009TruSeq Adapter, Index 14 (97% over 44bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTGAGATC1088790.18709857874400745No Hit
CGGGAAGCGGAGTTTGTAGTGAGTCGAGATTGCGTTATTGTAGTTCGTAGTTTGAGATCGGAAGAGCACACGTCT1078520.18533377340624632Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGATTGCGGATTGTAGTGGCGTAATTTCGGTTTATTGTAAGTTTCGTTTTTTGAGATCGGAAGAGCACACGTCT890860.15308612299882116Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGTGTGGTGGTGGGTGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGTGTGAATTTGAGATC835970.14365377976710653No Hit
TGGGTGTAGTGGTGGGTGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGTGTGAATTTGAGATC788070.13542260394639No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA773410.13290341735908928TruSeq Adapter, Index 14 (97% over 41bp)
CGGGATGGTTTCGATTTTTTGATTTCGTGATTCGTTCGTTTCGGTTTTTTAAAGTGTTGGGATTATAGGCGTGAG684260.11758380724600202No Hit
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTGAGATC614170.10553948337806837No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)