FastQCFastQC Report
Thu 12 May 2022
EGAF00004675346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58193387
Sequences flagged as poor quality0
Sequence length75
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAACTACGAACTACAATAACGCAATCTCGACTCACTACAAACTCCGCTTCCCGAGATCGGAAGAGCGTCGTGTA1013170.1741039750788178Illumina Single End PCR Primer 1 (100% over 21bp)
CAAATTCACGCCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAAACGCCCGCCACTACGCCCGAGATC953810.1639035033310572No Hit
CAAAAAACGAAACTTACAATAAACCGAAATTACGCCACTACAATCCGCAATCCGAGATCGGAAGAGCGTCGTGTA846270.1454237403298076Illumina Single End PCR Primer 1 (100% over 21bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA791540.13601889163110578Illumina Single End PCR Primer 1 (100% over 57bp)
CAAATTCACACCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAAACACCCACCACCACACCCAAGATC755640.12984980578635164No Hit
CAAATTCACACCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAAACACCCACCACTACACCCAAGATC700010.12029030033945266No Hit
CAAACGCGATAACTCACGCCTATAATCCCAACACTTTAAAAAACCGAAACGAACGAATCACGAAATCAAAAAATC649030.11152985475823911No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)