FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004675348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41337053
Sequences flagged as poor quality0
Sequence length75
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATTCACACCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAAACACCCACCACCACACCCAAGATC748220.18100467878056037No Hit
CAAATTCACACCATTCTCCTACCTCAACCTCCCAAATAACTAAAACTACAAACACCCACCACTACACCCAAGATC701830.16978230160722876No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA556550.13463707729721322Illumina Single End PCR Primer 1 (100% over 57bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTCAC13650.066.7278443
TATCGGA96800.066.452281
AAGTTCA83200.066.3230442
CAGATTC64000.066.1612851
AGTTCAC50250.065.707013
AGGTTCA16000.065.568152
TAGGTTC7650.065.410261
GCCATTC900050.063.95405610
AGATTCA69900.063.835272
GGTTCAA14600.063.803833
TAGGCTC6600.063.7903861
TCGGATA5450.063.3202861
ATTCACG905650.063.2159274
CAAGTTC83300.063.1363261
TCGGCCA10300.062.9883081
TTCACGC928700.062.787445
CGCCATT931850.062.6749239
TCGGCTA7050.062.655641
CAGGTTC11350.062.6340831
CGGTTCA11550.062.4458582
Produced by FastQC (version 0.11.5)