FastQCFastQC Report
Wed 11 May 2022
EGAF00004675349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004675349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51550876
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA13073172.535974364431751TruSeq Adapter, Index 16 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2142250.41556034857681173TruSeq Adapter, Index 16 (97% over 40bp)
TGGGTGTGGTGGTGGGTGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGTGTGAATTTGAGATC1863140.36141771868241385No Hit
TGGGTGTAGTGGTGGGTGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGTGTGAATTTGAGATC1699310.32963746338665517No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1192260.23127832008131155TruSeq Adapter, Index 16 (97% over 40bp)
CGGGAAGCGGAGTTTGTAGTGAGTCGAGATTGCGTTATTGTAGTTCGTAGTTTGAGATCGGAAGAGCACACGTCT735360.14264743047237452Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGAAGTGGAGTTTGTAGTGAGTTGAGATTGTGTTATTGTAGTTTGTAGTTTGAGATCGGAAGAGCACACGTCT705310.13681823757951272Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)